Paper-Based for Isolation of Extracellular Vesicles

Yi-Hsing Hsiao; Chihchen Chen

doi:10.1007/978-1-4939-7253-1_5

Paper-Based for Isolation of Extracellular Vesicles

Methods Mol Biol. 2017:1660:43-54. doi: 10.1007/978-1-4939-7253-1_5.

Authors

Yi-Hsing Hsiao^{1

2}, Chihchen Chen^{3

4}

Affiliations

¹ Institute of Nanoengineering and Microsystems, National Tsing Hua University, No.101, Section 2, Guangfu Rd., East Dist, Hsinchu, 30013, Taiwan.
² Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, 35053, Taiwan.
³ Institute of Nanoengineering and Microsystems, National Tsing Hua University, No.101, Section 2, Guangfu Rd., East Dist, Hsinchu, 30013, Taiwan. chihchen@mx.nthu.edu.tw.
⁴ Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, 30013, Taiwan. chihchen@mx.nthu.edu.tw.

PMID: 28828647
DOI: 10.1007/978-1-4939-7253-1_5

Abstract

Paper-based devices chemically functionalized with capturing molecules enable the isolation and characterization of extracellular vesicles (EVs) from samples of limited amount. Here, we describe the isolation of EV subpopulations from human serum samples. The morphology, content, and amount of captured EVs can be assessed using scanning electron microscopy (SEM ), transcriptome analysis, and paper-based enzyme-linked immunosorbent assays (pELISA), respectively. A colorimetric readout can be detected from 10 μL serum within 10 min.

Keywords: Cellulose paper; Chemical conjugation; Exosomes; Extracellular vesicles; Microfluidics; Paper ELISA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Fractionation / methods*
Enzyme-Linked Immunosorbent Assay / methods
Exosomes / chemistry
Exosomes / ultrastructure
Extracellular Vesicles* / chemistry
Extracellular Vesicles* / ultrastructure
Humans
Immunoprecipitation / methods
Paper*