Sulfated polysaccharide from Cyclocarya paliurus enhances the immunomodulatory activity of macrophages

Yue Yu; Mingyue Shen; Zhijun Wang; Yuanxing Wang; Mingyong Xie; Jianhua Xie

doi:10.1016/j.carbpol.2017.07.009

Sulfated polysaccharide from Cyclocarya paliurus enhances the immunomodulatory activity of macrophages

Carbohydr Polym. 2017 Oct 15:174:669-676. doi: 10.1016/j.carbpol.2017.07.009. Epub 2017 Jul 5.

Authors

Yue Yu¹, Mingyue Shen¹, Zhijun Wang¹, Yuanxing Wang¹, Mingyong Xie¹, Jianhua Xie²

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
² State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: jhxie@ncu.edu.cn.

PMID: 28821118
DOI: 10.1016/j.carbpol.2017.07.009

Abstract

In this study, a sulfated polysaccharide (S-CP_1-8) was obtained from Cyclocarya paliurus by chlorosulfonic acid-pyridine method. S-CP_1-8 was chemical characterized and cultured with macrophage RAW264.7 cells to evaluate its immunomodulatory activity. Preliminary physicochemical analysis identified arabinose (Ara), galactose (Gal) and glucose (Glc) were the major monosaccharides of S-CP_1-8, with average Molecular weight (Mw) of 970kDa and contained sulfate with degree of substitution (DS) of 0.12. Meanwhile, our findings demonstrated that tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 secretion levels were significantly increased by S-CP_1-8 treatment in RAW264.7 cells in a dose-dependent manner. Moreover, nitric oxide (NO) release and phagocytic activities of macrophages were significantly enhanced by the treatment of S-CP_1-8 compared with native Cyclocarya paliurus polysaccharide (CP). The results indicated that S-CP_1-8 could enhance the immunomodulatory effects on RAW264.7 macrophages, as comparison with CP group. S-CP_1-8 could serve as potential immunomodulatory agents to be used as complementary medicine or functional foods.

Keywords: Arabinose (PubChem CID: 66308); Chlorosulfonic acid (PubChem CID:24638); Cyclocarya paliurus; Fucose (PubChem CID:17106); Galactose (PubChem CID: 6036); Galacturonic acid (PubChem CID:439215); Glucose (PubChem CID:5793); Glucuronic acid (PubChem CID: 94715); Immune-enhancing; Immunomodulatory; Mannose (PubChem CID:18950); Polysaccharide; Pyridine (PubChem CID:1049); Rhamnose (PubChem CID: 25310); Sulfation; Xylose (PubChem CID:135191).

MeSH terms

Animals
Interleukin-1beta / metabolism
Interleukin-6 / metabolism
Juglandaceae / chemistry*
Macrophages / drug effects*
Mice
Nitric Oxide / metabolism
Phagocytosis
Polysaccharides / chemistry*
RAW 264.7 Cells
Sulfates
Tumor Necrosis Factor-alpha / metabolism

Substances

IL1B protein, mouse
Interleukin-1beta
Interleukin-6
Polysaccharides
Sulfates
Tumor Necrosis Factor-alpha
interleukin-6, mouse
Nitric Oxide