Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells

Abstract

The identification of therapeutically targetable mutations in circulating tumor cells (CTCs) from cancer patient blood is increasingly used to personalize patient care. Here, we describe a novel approach for the enumeration, capture, and molecular analysis of CTCs from blood using an FDA-approved CTC enrichment and enumeration platform followed by dielectrophoretic capture and next-generation sequencing.

Keywords: Circulating tumor cell; Liquid biopsy; Next-generation sequencing; Personalized medicine; Whole genome amplification.