Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells

Methods Mol Biol. 2017:1634:81-91. doi: 10.1007/978-1-4939-7144-2_6.

Abstract

Analysis of rare circulating tumor cells enriched from metastatic cancer patients yields critical information on disease progression, therapy response, and the mechanism of cancer metastasis. Here we describe in detail a label-free enrichment process of circulating tumor cells based on its unique physical properties (size and deformability). Viable circulating tumor cells can be successfully enriched and analyzed, or easily released for further characterization due to the novel separable two-layer design.

Keywords: CTC in vitro culture; Cancer metastasis; Circulating tumor cells; Microfabrication; Viable CTC isolation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / metabolism
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / immunology
  • Biomarkers, Tumor / metabolism
  • Cell Count
  • Cell Separation / instrumentation
  • Cell Separation / methods*
  • Cell Size
  • Dimethylpolysiloxanes / chemistry
  • Epithelial Cell Adhesion Molecule / genetics
  • Epithelial Cell Adhesion Molecule / immunology
  • Epithelial Cell Adhesion Molecule / metabolism
  • Equipment Design*
  • Filtration / instrumentation
  • Filtration / methods*
  • Fluorescent Antibody Technique / methods
  • Fluorescent Dyes / chemistry
  • Humans
  • Immunoconjugates / chemistry
  • Leukocyte Common Antigens / genetics
  • Leukocyte Common Antigens / immunology
  • Leukocyte Common Antigens / metabolism
  • Lymphatic Metastasis
  • Mice
  • Neoplasms / blood
  • Neoplasms / diagnosis*
  • Neoplasms / immunology
  • Neoplasms / pathology
  • Neoplastic Cells, Circulating / immunology
  • Neoplastic Cells, Circulating / metabolism
  • Neoplastic Cells, Circulating / pathology*
  • Polymers / chemistry
  • Protein Binding
  • Rheology
  • Xylenes / chemistry

Substances

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • Dimethylpolysiloxanes
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule
  • Fluorescent Dyes
  • Immunoconjugates
  • Polymers
  • Xylenes
  • parylene
  • baysilon
  • Leukocyte Common Antigens
  • PTPRC protein, human