Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF

Tuberculosis (Edinb). 2017 Sep:106:53-55. doi: 10.1016/j.tube.2017.06.005. Epub 2017 Jul 3.

Abstract

GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antitubercular / pharmacology
  • Automation, Laboratory
  • Bacteriological Techniques*
  • Biopsy
  • Case-Control Studies
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / isolation & purification
  • Drug Resistance, Bacterial / genetics
  • Humans
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Paraffin Embedding*
  • Predictive Value of Tests
  • Real-Time Polymerase Chain Reaction / methods*
  • Rifampin / pharmacology
  • Tuberculosis, Lymph Node / diagnosis*
  • Tuberculosis, Lymph Node / genetics
  • Tuberculosis, Lymph Node / pathology
  • Tuberculosis, Pleural / diagnosis*
  • Tuberculosis, Pleural / genetics
  • Tuberculosis, Pleural / pathology
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / genetics
  • Tuberculosis, Pulmonary / pathology

Substances

  • Antibiotics, Antitubercular
  • DNA, Bacterial
  • Rifampin