Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015

Euro Surveill. 2017 Aug 3;22(31):30586. doi: 10.2807/1560-7917.ES.2017.22.31.30586.

Abstract

Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.

Keywords: Escherichia coli; antimicrobial resistance; bovine; cattle; colistin; mcr-1; mcr-3.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / pharmacology*
  • Cattle / microbiology*
  • Colistin / administration & dosage
  • Colistin / pharmacology*
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / drug effects*
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary
  • Escherichia coli Proteins / genetics*
  • Feces / microbiology
  • Microbial Sensitivity Tests
  • Molecular Typing
  • Peptides
  • Polymerase Chain Reaction
  • Sentinel Surveillance
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Escherichia coli Proteins
  • MCR 4 peptide
  • MCR-1 protein, E coli
  • Peptides
  • beta-Lactamases
  • Colistin