Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent

Hamid Maadi; Babak Nami; Zhixiang Wang

doi:10.1007/978-1-4939-7219-7_6

Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent

Methods Mol Biol. 2017:1652:101-108. doi: 10.1007/978-1-4939-7219-7_6.

Authors

Hamid Maadi¹, Babak Nami², Zhixiang Wang³

Affiliations

¹ Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada. hmaadi@ualberta.ca.
² Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.
³ Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada. zhixiang.wang@ualberta.ca.

PMID: 28791636
DOI: 10.1007/978-1-4939-7219-7_6

Abstract

Dimerization of the epithelial growth factor (EGF) family of receptor tyrosine kinases is a crucial step for activation of these receptors. Different chemicals such as BS³ and DSS have been introduced to covalently bind the interacting receptors and fix the dimers. Unique properties of BS³ including higher water solubility and membrane impermeability make it suitable for assessing receptor-receptor interactions in live cells. In this protocol, we aim to explain a method to evaluate the dimerization of EGF receptors family using BS³ as a cross-linker reagent.

Keywords: BS3; Dimerization; EGF receptor family; Immunoblotting.

MeSH terms

Biological Assay / methods*
Blotting, Western
Cell Line
Cross-Linking Reagents*
Epidermal Growth Factor / metabolism
ErbB Receptors / chemistry
ErbB Receptors / metabolism*
Humans
Phosphorylation
Protein Multimerization*
Receptor Protein-Tyrosine Kinases / chemistry
Receptor Protein-Tyrosine Kinases / metabolism*

Substances

Cross-Linking Reagents
Epidermal Growth Factor
ErbB Receptors
Receptor Protein-Tyrosine Kinases