The occurrence of CTX-M-25-producing Enterobacteriaceae in day-old broiler chicks in Japan

J Vet Med Sci. 2017 Oct 7;79(10):1644-1647. doi: 10.1292/jvms.17-0294. Epub 2017 Aug 7.

Abstract

Day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between January and July 2016. Sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant Enterobacteriaceae. Cefotaxime (CTX)-resistant Klebsiella pneumoniae (1 isolate) and Enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. Conjugation experiments revealed that the blaCTX-M-25 gene conferring CTX resistance was transferred from the K. pneumoniae isolate and 2 of the 4 E. cloacae isolates to Escherichia coli DH5α via IncA/C plasmids carrying the gene. Our results suggested that the blaCTX-M-25 gene originating from chicks may be spread among commercial broiler farms.

Keywords: ESBL; Enterobacteriaceae; antimicrobial resistance; chick.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / therapeutic use
  • Cephalosporin Resistance*
  • Chickens / microbiology*
  • Enterobacter cloacae / drug effects
  • Enterobacter cloacae / enzymology
  • Enterobacter cloacae / metabolism
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / enzymology
  • Enterobacteriaceae / metabolism
  • Enterobacteriaceae Infections / drug therapy
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / veterinary*
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology
  • Escherichia coli / metabolism
  • Japan
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / metabolism
  • Microbial Sensitivity Tests / veterinary
  • Poultry Diseases / drug therapy
  • Poultry Diseases / microbiology*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • beta-Lactamases