Ability of an alkali-tolerant mutant strain of the microalga Chlorella sp. AT1 to capture carbon dioxide for increasing carbon dioxide utilization efficiency

Abstract

An alkali-tolerant Chlorella sp. AT1 mutant strain was screened by NTG mutagenesis. The strain grew well in pH 6-11 media, and the optimal pH for growth was 10. The CO₂ utilization efficiencies of Chlorella sp. AT1 cultured with intermittent 10% CO₂ aeration for 10, 20 and 30min at 3-h intervals were approximately 80, 42 and 30%, respectively. In alkaline medium (pH=11) with intermittent 10% CO₂ aeration for 30min at 3-, 6- and 12-h intervals, the medium pH gradually changed to 10, and the biomass productivities of Chlorella sp. AT1 were 0.987, 0.848 and 0.710gL^-1d^-1, respectively. When Chlorella sp. AT1 was aerated with 10% CO₂ intermittently for 30min at 3-h intervals in semi-continuous cultivation for 21days, the biomass concentration and biomass productivity were 4.35gL^-1 and 0.726gL^-1d^-1, respectively. Our results show that CO₂ utilization efficiency can be markedly increased by intermittent CO₂ aeration and alkaline media as a CO₂-capturing strategy for alkali-tolerant microalga cultivation.

Keywords: Alkali-tolerant; CO(2) capture; CO(2) utilization efficiency; Chlorella; Intermittent aeration.