Knowledge about the interactions of biomaterials with biological matrices is still poor however crucial to future development in terms of how to cope with immunomodulatory adverse effects and to control the integration of implants into tissue or the targeting of nano devices. The appropriate design of a material depends on reliable data about the influence of oligomer or polymer configuration and dispersity as well as surface properties including chemical modifications. This work addressed the interaction of four polylactide (PLA) resomers, a well-established, biodegradable biomaterial, and two physiologically relevant proteins, which are ubiquitous in human serum - albumin (HSA) and fibrinogen (HFG). Bovine serum albumin (BSA) was carried along as reference. The amount and stability of protein binding to plane material was assessed by surface plasmon resonance (SPR), the formation of protein corona around NPs by nanoparticle tracking analysis (NTA). The results demonstrated the particular value of SPR and NTA as techniques for the characterisation and prediction of the interactions of implants or NPs with matrix components. Both techniques are complementary with respect to a deeper understanding of changes in the layer composition with time, which is known as the Vroman effect, and, therefore, are considered of value for affecting the interaction processes by material design.
Keywords: Biomaterials; Corona; Nanoparticle tracking analysis; Protein binding; Surface plasmon resonance; Vroman.
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