ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP

FEBS Lett. 2017 Sep;591(18):2793-2802. doi: 10.1002/1873-3468.12780. Epub 2017 Aug 21.

Abstract

Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing 3 (ACBD3) is likely to be a novel binding partner of Golgin45. ACBD3 interacts with Golgin45 via its GOLD domain, while its co-expression significantly increases Golgin45 targeting to the Golgi. Furthermore, ACBD3 recruits TBC1D22, a Rab33b GTPase activating protein (GAP), to a large multi-protein complex containing Golgin45 and GRASP55. These results suggest that ACBD3 may provide a scaffolding to organize the Golgi stacking proteins and a Rab33b-GAP at the medial-Golgi.

Keywords: ACBD3; Golgi; Golgin45; Rab-GTPase; membrane trafficking.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / chemistry
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Fluorescent Antibody Technique
  • GTPase-Activating Proteins / chemistry
  • GTPase-Activating Proteins / metabolism*
  • Golgi Apparatus / metabolism*
  • HeLa Cells
  • Humans
  • Immunoblotting
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism*
  • Microscopy, Confocal
  • Protein Binding
  • Vesicular Transport Proteins / chemistry
  • Vesicular Transport Proteins / metabolism
  • rab GTP-Binding Proteins / chemistry
  • rab GTP-Binding Proteins / metabolism*

Substances

  • ACBD3 protein, human
  • Adaptor Proteins, Signal Transducing
  • GTPase-Activating Proteins
  • Membrane Proteins
  • Vesicular Transport Proteins
  • RAB33B protein, human
  • rab GTP-Binding Proteins