Highly Absorbent Antibacterial Hemostatic Dressing for Healing Severe Hemorrhagic Wounds

Ting-Ting Li; Ching-Wen Lou; An-Pang Chen; Mong-Chuan Lee; Tsing-Fen Ho; Yueh-Sheng Chen; Jia-Horng Lin

doi:10.3390/ma9090793

Highly Absorbent Antibacterial Hemostatic Dressing for Healing Severe Hemorrhagic Wounds

Materials (Basel). 2016 Sep 21;9(9):793. doi: 10.3390/ma9090793.

Authors

Ting-Ting Li¹, Ching-Wen Lou^{2

3}, An-Pang Chen⁴, Mong-Chuan Lee⁵, Tsing-Fen Ho⁶, Yueh-Sheng Chen⁷, Jia-Horng Lin^{8

9

10

11

12}

Affiliations

¹ Innovation Platform of Intelligent and Energy-Saving Textiles, School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China. tingtingli@tjpu.edu.cn.
² Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou 350108, China. cwlou@ctust.edu.tw.
³ Graduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan. cwlou@ctust.edu.tw.
⁴ Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan. duncannano@gmail.com.
⁵ Graduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan. mclee@ctust.edu.tw.
⁶ Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan. tfho@ctust.edu.tw.
⁷ Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40402, Taiwan. yuehsc@mail.cmu.edu.tw.
⁸ Innovation Platform of Intelligent and Energy-Saving Textiles, School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China. jhlin@fcu.edu.tw.
⁹ Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou 350108, China. jhlin@fcu.edu.tw.
¹⁰ Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan. jhlin@fcu.edu.tw.
¹¹ School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. jhlin@fcu.edu.tw.
¹² Department of Fashion Design, Asia University, Taichung 41354, Taiwan. jhlin@fcu.edu.tw.

Abstract

To accelerate healing of severe hemorrhagic wounds, a novel highly absorbent hemostatic dressing composed of a Tencel^®/absorbent-cotton/polylactic acid nonwoven base and chitosan/nanosilver antibacterial agent was fabricated by using a nonwoven processing technique and a freeze-drying technique. This study is the first to investigate the wicking and water-absorbing properties of a nonwoven base by measuring the vertical wicking height and water absorption ratio. Moreover, blood agglutination and hemostatic second tests were conducted to evaluate the hemostatic performance of the resultant wound dressing. The blending ratio of fibers, areal weight, punching density, and fiber orientation, all significantly influenced the vertical moisture wicking property. However, only the first two parameters markedly affected the water absorption ratio. After the nonwoven base absorbed blood, scanning electron microscope (SEM) observation showed that erythrocytes were trapped between the fibrin/clot network and nonwoven fibers when coagulation pathways were activated. Prothrombin time (PT) and activated partial thromboplastin time (APTT) blood agglutination of the resultant dressing decreased to 14.34 and 50.94 s, respectively. In the femoral artery of the rate bleeding model, hemostatic time was saved by 87.2% compared with that of cotton cloth. Therefore, the resultant antibacterial wound dressing demonstrated greater water and blood absorption, as well as hemostatic performance, than the commercially available cotton cloth, especially for healing severe hemorrhagic wounds.

Keywords: fiber technology; hemostatic; nanosilver; nonwoven; wound dressing.