High resolution mass spectrometry studies of sulforaphane and indole-3-carbinol in broccoli

Maroula G Kokotou; Panagiota-Kyriaki Revelou; Christos Pappas; Violetta Constantinou-Kokotou

doi:10.1016/j.foodchem.2017.05.139

High resolution mass spectrometry studies of sulforaphane and indole-3-carbinol in broccoli

Food Chem. 2017 Dec 15:237:566-573. doi: 10.1016/j.foodchem.2017.05.139. Epub 2017 May 31.

Authors

Maroula G Kokotou¹, Panagiota-Kyriaki Revelou¹, Christos Pappas¹, Violetta Constantinou-Kokotou²

Affiliations

¹ Chemical Laboratories, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
² Chemical Laboratories, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece. Electronic address: vikon@aua.gr.

PMID: 28764036
DOI: 10.1016/j.foodchem.2017.05.139

Abstract

Broccoli is a rich source of bioactive compounds. Among them, sulforaphane and indole-3-carbinol have attracted a lot of attention, since their consumption is associated with reduced risk of cancer. In this work, the development of an efficient and direct method for the simultaneous determination of sulforaphane and indole-3-carbinol in broccoli using UPLC-HRMS/MS is described. The correlation coefficient, and limits of detection (LOD) and quantification (LOQ) were 0.993, 0.77mg/L and 2.35mg/L for sulforaphane and 0.997, 0.42mg/L, 1.29mg/L for indole-3-carbinol, respectively. The content of sulforaphane and indole-3-carbinol varied between 72±9-304±2mg and 77±1-117±3mg per 100g of fresh florets, respectively. Taking into consideration the differences in cultivar, geography, season and environmental factors, the results agreed with values published in the literature using other techniques.

Keywords: Broccoli; High resolution mass spectrometry; Indole-3-carbinol; Sulforaphane.

MeSH terms

Brassica*
Humans
Indoles / analysis*
Isothiocyanates / analysis*
Mass Spectrometry
Sulfoxides
Thiocyanates

Substances

Indoles
Isothiocyanates
Sulfoxides
Thiocyanates
indole-3-carbinol
sulforaphane