DNA Damage Tolerance by Eukaryotic DNA Polymerase and Primase PrimPol

Elizaveta O Boldinova; Paulina H Wanrooij; Evgeniy S Shilkin; Sjoerd Wanrooij; Alena V Makarova

doi:10.3390/ijms18071584

DNA Damage Tolerance by Eukaryotic DNA Polymerase and Primase PrimPol

Int J Mol Sci. 2017 Jul 21;18(7):1584. doi: 10.3390/ijms18071584.

Authors

Elizaveta O Boldinova¹, Paulina H Wanrooij², Evgeniy S Shilkin³, Sjoerd Wanrooij⁴, Alena V Makarova⁵

Affiliations

¹ Institute of Molecular Genetics of Russian Academy of Sciences, Kurchatov sq. 2, 123182 Moscow, Russia. lizaboldinova@yandex.ru.
² Department of Medical Biochemistry and Biophysics, Umeå University, 901 87 Umeå, Sweden. paulina.wanrooij@umu.se.
³ Institute of Molecular Genetics of Russian Academy of Sciences, Kurchatov sq. 2, 123182 Moscow, Russia. shilkinevgeniy.chem@gmail.com.
⁴ Department of Medical Biochemistry and Biophysics, Umeå University, 901 87 Umeå, Sweden. sjoerd.wanrooij@umu.se.
⁵ Institute of Molecular Genetics of Russian Academy of Sciences, Kurchatov sq. 2, 123182 Moscow, Russia. amakarova-img@yandex.ru.

Abstract

PrimPol is a human deoxyribonucleic acid (DNA) polymerase that also possesses primase activity and is involved in DNA damage tolerance, the prevention of genome instability and mitochondrial DNA maintenance. In this review, we focus on recent advances in biochemical and crystallographic studies of PrimPol, as well as in identification of new protein-protein interaction partners. Furthermore, we discuss the possible functions of PrimPol in both the nucleus and the mitochondria.

Keywords: DNA damage; PrimPol; mitochondria; replication.

Publication types

Review

MeSH terms

Cell Nucleus / genetics
Cell Nucleus / metabolism
Crystallography
DNA Damage*
DNA Primase / chemistry*
DNA Primase / metabolism*
DNA Replication
DNA, Mitochondrial / genetics
DNA-Directed DNA Polymerase / chemistry*
DNA-Directed DNA Polymerase / metabolism*
Genomic Instability
Humans
Mitochondria / genetics
Mitochondria / metabolism
Multifunctional Enzymes / chemistry*
Multifunctional Enzymes / metabolism*

Substances

DNA, Mitochondrial
Multifunctional Enzymes
DNA Primase
PrimPol protein, human
DNA-Directed DNA Polymerase