DNA Preparation from Schizosaccharomyces pombe

Assen Roguev; Jiewei Xu; Nevan J Krogan

doi:10.1101/pdb.prot091959

DNA Preparation from Schizosaccharomyces pombe

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot091959.

Authors

Assen Roguev¹, Jiewei Xu², Nevan J Krogan²

Affiliations

¹ Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518 roguev@gmail.com.
² Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518.

PMID: 28733411
DOI: 10.1101/pdb.prot091959

Abstract

This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

MeSH terms

DNA, Fungal / isolation & purification*
Genetics, Microbial / methods*
Genotyping Techniques
Molecular Biology / methods*
Mycological Typing Techniques
Polymerase Chain Reaction
Schizosaccharomyces / genetics*

Substances

DNA, Fungal