Oxygen and Indocyanine Green loaded microparticles for dual-mode imaging and sonodynamic treatment of cancer cells

Rong Ma; Qiang Wu; Ting Si; Shufang Chang; Ronald X Xu

doi:10.1016/j.ultsonch.2017.03.019

Oxygen and Indocyanine Green loaded microparticles for dual-mode imaging and sonodynamic treatment of cancer cells

Ultrason Sonochem. 2017 Nov:39:197-207. doi: 10.1016/j.ultsonch.2017.03.019. Epub 2017 Apr 20.

Authors

Rong Ma¹, Qiang Wu², Ting Si³, Shufang Chang⁴, Ronald X Xu⁵

Affiliations

¹ Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230027, China.
² Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230027, China.
³ Department of Modern Mechanics, University of Science and Technology of China, Hefei, Anhui 230027, China.
⁴ Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address: shfch2005@163.com.
⁵ Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230027, China; Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA. Electronic address: xu.202@osu.edu.

PMID: 28732936
DOI: 10.1016/j.ultsonch.2017.03.019

Abstract

Oxygen and Indocyanine Green (ICG) loaded microparticles (OI-MPs) were fabricated by a gas-driven coaxial flow focusing (CFF) process for dual-mode imaging and sonodynamic therapy (SDT). The produced OI-MPs agent showed stable optical properties, superior imaging depth in near infrared (NIR) fluorescence imaging, and enhanced acoustic contrast after ultrasound mediation. We hypothesized that encapsulating ICG and oxygen in microparticles would enhance reactive oxygen species (ROS) production in SDT. This hypothesis was validated in a cell-free environment. We further hypothesized that ultrasound mediated fragmentation of the OI-MPs would induce cytotoxicity and apoptosis of cancer cells. This hypothesis was validated in SKOV3 ovarian cancer cells. Our research demonstrated that OI-MPs can be potentially used as a dual-mode theranostic agent for image guided SDT with enhanced efficacy. Further study is needed to delineate the mechanism of ROS-induced cell apoptosis and optimize the OI-MPs formulation for the maximal anti-cancer potency.

Keywords: Coaxial flow focusing; Indocyanine Green; Multifunctional microparticles; Oxygen; Sonodynamic therapy; Ultrasonic image.

MeSH terms

Apoptosis / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Humans
Indocyanine Green / chemistry*
Microspheres*
Molecular Imaging / methods*
Oxygen / chemistry*
Ultrasonic Waves*

Substances

Indocyanine Green
Oxygen