Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A

Yang Liu; Meng Cai; Weiping Wu; Yuting Fang; Pei She; Shouming Xu; Jianguo Li; Kang Zhao; Jingjuan Xu; Ning Bao; Anping Deng

doi:10.1016/j.bios.2017.04.002

Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A

Biosens Bioelectron. 2018 Jan 15:99:21-27. doi: 10.1016/j.bios.2017.04.002. Epub 2017 Apr 11.

Authors

Yang Liu¹, Meng Cai², Weiping Wu², Yuting Fang², Pei She³, Shouming Xu³, Jianguo Li⁴, Kang Zhao³, Jingjuan Xu⁵, Ning Bao², Anping Deng⁶

Affiliations

¹ The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, 199 Renai Rd., Suzhou 215123, China; School of Public Health, Nantong University, 9 Seyuan Rd., Nantong 226019, China.
² School of Public Health, Nantong University, 9 Seyuan Rd., Nantong 226019, China.
³ The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, 199 Renai Rd., Suzhou 215123, China.
⁴ The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, 199 Renai Rd., Suzhou 215123, China. Electronic address: lijgsd@suda.edu.cn.
⁵ State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
⁶ The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, 199 Renai Rd., Suzhou 215123, China. Electronic address: denganping@suda.edu.cn.

PMID: 28732345
DOI: 10.1016/j.bios.2017.04.002

Abstract

Herein, we reported a disposable electroanalytical device for competitive enzyme-linked immunosorbent assay (ELISA) of phenylethanolamine A (PA). Conductive carbon tape coated with gold cluster served as the working electrode, while the counter and reference electrodes were fabricated on the filter paper by the screen-printing technique. Separating fabrication of the working electrode from other electrodes could make it possible for full utilization of the working electrode modification in bulk and no contamination of the immune-reagents on the counter and reference electrodes. The gold cluster played an important role in both immobilizing antigen and accelerating electron transfer. The eight-channel devices were utilized to detect PA on the strategy of competitive ELISA. The detection range and the limit of detection (LOD) using differential pulse voltammetry for PA were 0.005-60ngmL^-1 and 2.6pgmL^-1, respectively. And also, the linear range for PA performed by square wave voltammetry was 0.05-60ngmL^-1 with the LOD value as 0.028ngmL^-1. The results clearly demonstrated that the proposed electroanalytical devices could be successfully applied in immunoassay and might be further developed for determination of different analytes based on ELISA format.

Keywords: Disposable devices; Electrochemical ELISA; Multichannel; Phenylethanolamine A; Screen printing.

MeSH terms

2-Hydroxyphenethylamine / analogs & derivatives*
2-Hydroxyphenethylamine / isolation & purification
Biosensing Techniques*
Carbon / chemistry
Electrochemistry
Enzyme-Linked Immunosorbent Assay*
Gold / chemistry
Limit of Detection

Substances

2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol
Carbon
Gold
2-Hydroxyphenethylamine