De novo transcriptome assembly for the lung of the ornamented pygmy frog (Microhyla fissipes)

Lusha Liu; Shouhong Wang; Lanying Zhao; Jianping Jiang

doi:10.1016/j.gdata.2017.07.002

De novo transcriptome assembly for the lung of the ornamented pygmy frog (Microhyla fissipes)

Genom Data. 2017 Jul 4:13:44-45. doi: 10.1016/j.gdata.2017.07.002. eCollection 2017 Sep.

Authors

Lusha Liu¹, Shouhong Wang^{1

2}, Lanying Zhao³, Jianping Jiang¹

Affiliations

¹ Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
² University of Chinese Academy of Sciences, Beijing 100049, China.
³ Sichuan University, Chengdu 610041, China.

Abstract

Microhyla fissipes, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. M. fissipes use of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in M. fissipes is very limited. In this study, we conducted de novo transcriptome assembly for the lung of M. fissipes using the Illumina HiSeq4000 platform. We obtained approximately 9.0 GB clean data from the lung of the stage 28 tadpole with lung inflation (NCBI accession numbers: SRP107055). De novo transcriptome assembly identified 209,358 transcripts and 93,813 unigenes. In addition, BLASTX against NR, NT, KO, SwissProt, PFAM, GO and KOG databases were used to annotate all the 93,813 unigenes. This study provides the transcriptome and functional annotation of genes in M. fissipes lung development, which will be useful for comparative transcriptome analyses and promote research into mechanism of lung development in anuran.

Keywords: Lung; Microhyla fissipes; Transcriptome; Unigenes.