Transduction of a Neospora caninum antigen gene into mammalian cells using a modified Bombyx mori nucleopolyhedrovirus for antibody production

Tatsuya Kato; Kohei Itagaki; Mai Yoshimoto; Rikito Hiramatsu; Hamizah Suhaimi; Tetsuya Kohsaka; Enoch Y Park

doi:10.1016/j.jbiosc.2017.06.015

Transduction of a Neospora caninum antigen gene into mammalian cells using a modified Bombyx mori nucleopolyhedrovirus for antibody production

J Biosci Bioeng. 2017 Dec;124(6):606-610. doi: 10.1016/j.jbiosc.2017.06.015. Epub 2017 Jul 14.

Authors

Tatsuya Kato¹, Kohei Itagaki², Mai Yoshimoto², Rikito Hiramatsu², Hamizah Suhaimi³, Tetsuya Kohsaka², Enoch Y Park⁴

Affiliations

¹ Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Division of Applied Biological Chemistry, College of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
² Laboratory of Biotechnology, Division of Applied Biological Chemistry, College of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
³ Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
⁴ Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Division of Applied Biological Chemistry, College of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: park.enoch@shizuoka.ac.jp.

PMID: 28716628
DOI: 10.1016/j.jbiosc.2017.06.015

Abstract

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) can easily enter and transduce foreign genes into mammalian cells, but these functions are difficult for Bombyx mori nucleopolyhedrovirus (BmNPV). In this study, we investigated the induction of antibody production in mice immunized with an engineered BmNPV. The GP64 of BmNPV (BmGP64) was replaced with the GP64 of AcMNPV (AcGP64); this construct, designated BmNPVΔbgp/AcGP64, displays AcGP64 on the surface of BmNPV. The Neospora caninum antigen (NcSRS2) expression cassette, consisting of the cytomegalovirus immediate-early promoter and NcSRS2 from N. caninum, was inserted into BmNPVΔbgp/AcGP64; this construct was designated BmNPVΔbgp/AcGP64/SRS2. For comparison, AcMNPV/SRS2, which contains the same NcSRS2 expression cassette as for BmNPVΔbgp/AcGP64, was also constructed. NcSRS2 was expressed in HEK293T cells when the engineered BmNPVs were transduced at a multiplicity of infection of 150. BmNPVΔbgp/AcGP64/SRS2 induced the production of NcSRS2-specific antibodies in mice, whereas AcMNPV/SRS2 and the control BmNPV did not. These results suggest that BmNPV prepared from silkworm hemolymph induces the production of antigen-specific antibodies in immunized mice and can be used for antibody production and vaccine development.

Keywords: AcMNPV; Antibody production; BmNPV; Silkworm; Transduction.

MeSH terms

Animals
Antibody Formation / genetics
Antibody Formation / immunology*
Antigens, Protozoan / genetics*
Antigens, Protozoan / immunology*
Bombyx / virology
Female
HEK293 Cells
Hemolymph / immunology
Humans
Immunization
Mice
Neospora / genetics*
Neospora / immunology
Nucleopolyhedroviruses / genetics*
Nucleopolyhedroviruses / immunology*

Substances

Antigens, Protozoan

Supplementary concepts

Bombyx mori nucleopolyhedrovirus