Isolation and Characterization of Aphidicolin Derivatives from Tolypocladium inflatum

Jie Lin; Shubin Niu; Zhengfeng Ding; Renlei Wang; Qun Dai; Wei Wei; Rongrong Luo; Ling Liu

doi:10.3390/molecules22071168

Isolation and Characterization of Aphidicolin Derivatives from Tolypocladium inflatum

Molecules. 2017 Jul 12;22(7):1168. doi: 10.3390/molecules22071168.

Authors

Jie Lin¹, Shubin Niu², Zhengfeng Ding³, Renlei Wang⁴, Qun Dai⁵, Wei Wei⁶, Rongrong Luo⁷, Ling Liu⁸

Affiliations

¹ Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry,Jiangsu Second Normal University, Nanjing 210003, China. inasianskyjie@aliyun.com.
² School of Biomedicine, Beijing City University, Beijing 100083, China. niushubin@bcu.edu.cn.
³ Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry,Jiangsu Second Normal University, Nanjing 210003, China. ding@jssnu.edu.cn.
⁴ Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry,Jiangsu Second Normal University, Nanjing 210003, China. wrl3501988@163.com.
⁵ Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry,Jiangsu Second Normal University, Nanjing 210003, China. njdaiqun@163.com.
⁶ Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry,Jiangsu Second Normal University, Nanjing 210003, China. iamwei.w@163.com.
⁷ Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry,Jiangsu Second Normal University, Nanjing 210003, China. 18761602885@163.com.
⁸ State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. liul@im.ac.cn.

Abstract

Inflatin G (1), a new aphidicolin analogue, together with seven known compounds inflatin A (2), inflatin B (3), aphidicolin (4), aphidicolin-17-monoacetate (5), gulypyrone A (6), pyridoxatin rotamers A (7) and B (8), were isolated from the ascomycete fungus Tolypocladium inflatum. Their structures were determined through NMR analyses and the circular dichroism data of the in situ formed [Rh₂(OCOCF₃)₄] complexes. Compounds 1, 4, 5, 7, and 8 showed modest cytotoxicity against four human cancer cell lines A549, CNE1-MP1, A375, and MCF-7.

Keywords: Tolypocladium inflatum; biological activities; structure elucidation.

MeSH terms

Antineoplastic Agents / chemistry
Antineoplastic Agents / isolation & purification*
Antineoplastic Agents / pharmacology
Aphidicolin / analogs & derivatives*
Aphidicolin / chemistry
Aphidicolin / isolation & purification*
Aphidicolin / pharmacology
Cell Line, Tumor
Cell Proliferation / drug effects
Drug Screening Assays, Antitumor
Humans
Hypocreales / chemistry*

Substances

Antineoplastic Agents
inflatin B
inflatin G
Aphidicolin