Exercise-intensity dependent alterations in plasma redox status do not reflect skeletal muscle redox-sensitive protein signaling

Lewan Parker; Adam Trewin; Itamar Levinger; Christopher S Shaw; Nigel K Stepto

doi:10.1016/j.jsams.2017.06.017

Exercise-intensity dependent alterations in plasma redox status do not reflect skeletal muscle redox-sensitive protein signaling

J Sci Med Sport. 2018 Apr;21(4):416-421. doi: 10.1016/j.jsams.2017.06.017. Epub 2017 Jul 1.

Authors

Lewan Parker¹, Adam Trewin², Itamar Levinger³, Christopher S Shaw⁴, Nigel K Stepto⁵

Affiliations

¹ Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia. Electronic address: lewan.parker@vu.edu.au.
² Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia.
³ Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia; Australian Institute for Musculoskeletal Science (AIMSS), Australia.
⁴ Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia; Institute for Physical Activity and Nutrition, School of Exercise and Nutrition Sciences, Deakin University, Australia.
⁵ Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia; Australian Institute for Musculoskeletal Science (AIMSS), Australia; Monash Centre for Health Research and Implementation (MCHRI), School of Public Health and Preventative Medicine, Monash University and Monash Health, Australia.

PMID: 28689678
DOI: 10.1016/j.jsams.2017.06.017

Abstract

Objectives: Redox homeostasis and redox-sensitive protein signaling play a role in exercise-induced adaptation. The effects of sprint-interval exercise (SIE), high-intensity interval exercise (HIIE) and continuous moderate-intensity exercise (CMIE), on post-exercise plasma redox status are unclear. Furthermore, whether post-exercise plasma redox status reflects skeletal muscle redox-sensitive protein signaling is unknown.

Design: In a randomized crossover design, eight healthy adults performed a cycling session of HIIE (5×4min at 75% W_max), SIE (4×30s Wingate's), and CMIE work-matched to HIIE (30min at 50% of W_max).

Methods: Plasma hydrogen peroxide (H₂O₂), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD) activity, and catalase activity were measured immediately post, 1h, 2h and 3h post-exercise. Plasma redox status biomarkers were correlated with phosphorylation of skeletal muscle p38-MAPK, JNK, NF-κB, and IκBα protein content immediately and 3h post-exercise.

Results: Plasma catalase activity was greater with SIE (56.6±3.8Uml^-1) compared to CMIE (42.7±3.2, p<0.01) and HIIE (49.0±5.5, p=0.07). Peak plasma H₂O₂ was significantly (p<0.05) greater after SIE (4.6±0.6nmol/ml) and HIIE (4.1±0.4) compared to CMIE (3.3±0.5). Post-exercise plasma TBARS and SOD activity significantly (p<0.05) decreased irrespective of exercise protocol. A significant positive correlation was detected between plasma catalase activity and skeletal muscle p38-MAPK phosphorylation 3h post-exercise (r=0.40, p=0.04). No other correlations were detected (all p>0.05).

Conclusions: Low-volume SIE elicited greater post-exercise plasma catalase activity compared to HIIE and CMIE, and greater H₂O₂ compared to CMIE. Plasma redox status did not, however, adequately reflect skeletal muscle redox-sensitive protein signaling.

Keywords: Antioxidant defense; High-intensity exercise; Oxidative stress; Redox biology; Skeletal muscle adaptation.

Publication types

Randomized Controlled Trial

MeSH terms

Adaptation, Physiological*
Adult
Biomarkers / blood
Catalase / blood
Cross-Over Studies
Exercise / physiology*
Female
High-Intensity Interval Training*
Humans
Hydrogen Peroxide / blood
Male
Muscle, Skeletal / physiology*
Oxidation-Reduction
Phosphorylation
Superoxide Dismutase / blood
Thiobarbituric Acid Reactive Substances / analysis
Young Adult

Substances

Biomarkers
Thiobarbituric Acid Reactive Substances
Hydrogen Peroxide
Catalase
Superoxide Dismutase