Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles

Florian Puhm; Christoph J Binder

doi:10.1007/978-1-4939-7180-0_11

Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles

Methods Mol Biol. 2017:1643:147-154. doi: 10.1007/978-1-4939-7180-0_11.

Authors

Florian Puhm^{1

2}, Christoph J Binder^{3

4}

Affiliations

¹ Department of Laboratory Medicine, Medical University of Vienna, Lazarettgasse 14, AKH BT 25.2/6, 1090, Vienna, Austria.
² Research Center for Molecular Medicine (CeMM) of the Austrian Academy of Sciences, 1090, Vienna, Austria.
³ Department of Laboratory Medicine, Medical University of Vienna, Lazarettgasse 14, AKH BT 25.2/6, 1090, Vienna, Austria. christoph.binder@meduniwien.ac.at.
⁴ Research Center for Molecular Medicine (CeMM) of the Austrian Academy of Sciences, 1090, Vienna, Austria. christoph.binder@meduniwien.ac.at.

PMID: 28667535
DOI: 10.1007/978-1-4939-7180-0_11

Abstract

Natural IgM antibodies specific for oxidation-specific epitopes (OSEs) have been found to bind to circulating microvesicles (MVs), also known as microparticles. This chapter describes how endogenous natural IgM antibodies bound to circulating MV subsets can be characterized on the one hand, and how monoclonal natural IgM antibodies can be used to characterize subsets of circulating MVs on the other hand.

Keywords: Flow cytometry; Microparticles; Microvesicles; Natural IgM antibodies; Oxidation-specific epitopes.

MeSH terms

Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / metabolism
Cell-Derived Microparticles / metabolism*
Epitopes / immunology*
Epitopes / metabolism*
Flow Cytometry
Humans
Immunoglobulin M / immunology*
Immunoglobulin M / metabolism*
Oxidation-Reduction

Substances

Antibodies, Monoclonal
Epitopes
Immunoglobulin M