From Image Tiles to Web-Based Interactive Measurements in One Stop

Micros Today. 2015 Aug;21(Suppl 3):89-90. doi: 10.1017/S1431927615001245. Epub 2015 Sep 23.

Abstract

This article introduces readers to a web-based solution useful for interactive nanoscale measurements of centimeter-sized specimens. This solution is a client-server system that promotes collaborative measurements and discovery. The system consists of multiple computational modules that enable uploading microscopy images, extracting metadata, assembling many nanometer-resolution images into an image covering a centimeter-sized area, and interactive viewing and measuring of objects of interest at multiple length scales over terabyte-sized images. We illustrate the use of the system on images of aerosolized nanoparticles and dye particles on printing paper.