Bioactivity-guided isolation of chemical constituents against H2O2-induced neurotoxicity on PC12 from Cimicifuga dahurica (Turcz.) Maxim

Bioorg Med Chem Lett. 2017 Aug 1;27(15):3305-3309. doi: 10.1016/j.bmcl.2017.06.020. Epub 2017 Jun 8.

Abstract

Three new compounds (1, 6, 9), with six known compounds (2-5, 7-8) were obtained from water-soluble extract of Cimicifuga dahurica (Turcz.) Maxim. by bioactivity-guided isolation. Their structures were elucidated by chemical and spectral analysis, including 1D, 2D NMR data and HRESIMS. H2O2-induced neurotoxicity on PC12 cells model were conducted to evaluate the neuro-protective capability of these compounds. The piscidic acid derivatives compounds 4-7 showed marked neuro-protective effect at certain concentration.

Keywords: Cimicifuga dahurica (Turcz.) Maxim.; Cyclolanostane triterpenes; Neuro-protective; Piscidic acid derivatives.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Cimicifuga / chemistry*
  • Hydrogen Peroxide / metabolism
  • Hydroxybenzoates / chemistry
  • Hydroxybenzoates / isolation & purification
  • Hydroxybenzoates / pharmacology
  • Neurons / cytology
  • Neurons / drug effects*
  • Neurons / metabolism
  • Neuroprotective Agents / chemistry*
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • PC12 Cells
  • Rats
  • Triterpenes / chemistry*
  • Triterpenes / isolation & purification
  • Triterpenes / pharmacology*

Substances

  • Hydroxybenzoates
  • Neuroprotective Agents
  • Triterpenes
  • piscidic acid
  • Hydrogen Peroxide