Thaumasiovibrio occultus gen. nov. sp. nov. and Thaumasiovibrio subtropicus sp. nov. within the family Vibrionaceae, isolated from coral reef seawater off Ishigaki Island, Japan

A K M Rohul Amin; Mami Tanaka; Nurhidayu Al-Saari; Gao Feng; Sayaka Mino; Yoshitoshi Ogura; Tetsuya Hayashi; Pedro M Meirelles; Fabiano L Thompson; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe

doi:10.1016/j.syapm.2017.04.003

Thaumasiovibrio occultus gen. nov. sp. nov. and Thaumasiovibrio subtropicus sp. nov. within the family Vibrionaceae, isolated from coral reef seawater off Ishigaki Island, Japan

Syst Appl Microbiol. 2017 Jul;40(5):290-296. doi: 10.1016/j.syapm.2017.04.003. Epub 2017 Jun 16.

Authors

Affiliations

¹ Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University, Hakodate, Japan.
² Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University, Hakodate, Japan; Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM), Serdang, Malaysia.
³ Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka-shi, Fukuoka, Japan.
⁴ Institute of Biology, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
⁵ JapangCIAD, AC Mazatlan Unit for Aquaculture and Environmental Management, AP 711 Mazatlán, Sinaloa, Mexico.
⁶ Department of Food and Nutrition, Hakodate Junior College, Hakodate, Japan.
⁷ Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University, Hakodate, Japan. Electronic address: sawabe@fish.hokudai.ac.jp.

PMID: 28648725
DOI: 10.1016/j.syapm.2017.04.003

Abstract

Two phylogenetically distinct Vibrionaceae strains C4II189^T and C4V358^T isolated from reef seawater off Ishigaki Island, Japan, in 2014 were studied with advanced genome-based taxonomy approaches. All aspects of phylogenetic (16S rRNA phylogeny, MLSA), phenotypic and genetic (ANI, DDH, AAI, and the number of core genes) cohesions between the two identified species were high enough to propose them as members of a new genus within the family Vibrionaceae. Consequently, an eighth genus Thaumasiovibrio gen. nov. is proposed that contains two new species Thaumasiovibrio occultus sp. nov. strain C4II189^T (=DSM 101554^T=JCM 31629^T) (type species) and Thaumasiovibrio subtropicus sp. nov. strain C4V358^T (=DSM 101555^T=JCM 31630^T). Thaumasiovibrio species were phylogenetically distinct from the other Vibrionaceae species based on pyrH gene sequences. The combination of catalase negative, sensitivity to vibriostatic agent O/129, and green colony formation on TCBS for the phylogenetically affiliated strains was the diagnostic features for the current tentative identification of this genus.

Keywords: Coral reef; Genomic taxonomy; New genus; Seawater; Thaumasiovibrio; Vibrionaceae.

MeSH terms

Animals
Anthozoa / microbiology*
Base Composition / genetics
Coral Reefs*
DNA, Bacterial / genetics
Japan
Multilocus Sequence Typing
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Vibrionaceae* / classification
Vibrionaceae* / genetics
Vibrionaceae* / isolation & purification

Substances

DNA, Bacterial
RNA, Ribosomal, 16S