Genome Editing of Medaka

Satoshi Ansai; Masato Kinoshita

doi:10.1007/978-1-4939-7128-2_15

Genome Editing of Medaka

Methods Mol Biol. 2017:1630:175-188. doi: 10.1007/978-1-4939-7128-2_15.

Authors

Satoshi Ansai^{1

2}, Masato Kinoshita³

Affiliations

¹ Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Kyoto, Japan.
² Division of Ecological Genetics, Department of Population Genetics, National Institute of Genetics, Yata 1111, Mishima, 411-8540, Shizuoka, Japan.
³ Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Kyoto, Japan. kinoshit@kais.kyoto-u.ac.jp.

PMID: 28643259
DOI: 10.1007/978-1-4939-7128-2_15

Abstract

Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.

Keywords: CRISPR/Cas; Genome editing; Heteroduplex mobility assay; Medaka; Targeted integration; Targeted mutagenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electrophoresis, Microchip / methods*
Gene Editing / methods*
Gene Knockout Techniques
Genetic Vectors / administration & dosage
Models, Animal
Oryzias / genetics*
Plasmids / genetics