Hydrogel-Based In Vitro Models of Tumor Angiogenesis

Laura J Bray; Marcus Binner; Uwe Freudenberg; Carsten Werner

doi:10.1007/978-1-4939-7021-6_4

Hydrogel-Based In Vitro Models of Tumor Angiogenesis

Methods Mol Biol. 2017:1612:39-63. doi: 10.1007/978-1-4939-7021-6_4.

Authors

Laura J Bray^{1

2}, Marcus Binner³, Uwe Freudenberg³, Carsten Werner^{3

4}

Affiliations

¹ Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Saxony, Germany. laura.bray@qut.edu.au.
² Science and Engineering Faculty and Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD, 4059, Australia. laura.bray@qut.edu.au.
³ Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Saxony, Germany.
⁴ Technische Universität Dresden, Center for Regenerative Therapies Dresden, Dresden, Germany.

PMID: 28634934
DOI: 10.1007/978-1-4939-7021-6_4

Abstract

In situ forming hydrogels prepared from multi-armed poly(ethylene glycol) (PEG), glycosaminoglycans (GAG) and various peptides enable the development of advanced three dimensional (3D) culture models. Herein, we report methods for the PEG-GAG gel-based 3D co-cultivation of human umbilical vein endothelial cells, mesenchymal stromal cells, and different cancer cell lines. The resulting constructs allow for the exploration of interactions between solid tumors with 3D vascular networks in vitro to study the mechanistic aspects of cancer development and to perform drug testing.

Keywords: 3D model; Angiogenesis; Hydrogel; Tumor.

MeSH terms

Animals
Cell Line, Tumor
Coculture Techniques / methods*
Glycosaminoglycans / chemistry
Human Umbilical Vein Endothelial Cells
Humans
Hydrogels / chemistry*
MCF-7 Cells
Mesenchymal Stem Cells / cytology
Mice
Models, Biological*
Neovascularization, Pathologic*
Polyethylene Glycols / chemistry
Tissue Engineering

Substances

Glycosaminoglycans
Hydrogels
Polyethylene Glycols