Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants

Tom Schreiber; Alain Tissier

doi:10.1007/978-1-4939-7125-1_13

Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants

Methods Mol Biol. 2017:1629:185-204. doi: 10.1007/978-1-4939-7125-1_13.

Authors

Tom Schreiber¹, Alain Tissier²

Affiliations

¹ Department of Cell and Metabolic Biology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Saale, Germany.
² Department of Cell and Metabolic Biology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Saale, Germany. alain.tissier@ipb-halle.de.

PMID: 28623587
DOI: 10.1007/978-1-4939-7125-1_13

Abstract

Transcription factors with programmable DNA-binding specificity constitute valuable tools for the design of orthogonal gene regulatory networks for synthetic biology. Transcription activator-like effectors (TALEs), as natural transcription regulators, were used to design, build, and test libraries of synthetic TALE-activated promoters (STAPs) that show a broad range of expression levels in plants. In this chapter, we present protocols for the construction of artificial TALEs and corresponding STAPs.

Keywords: Designer TALE (dTALE); Golden Gate-based cloning; Synthetic TALE-activated promoter (STAP); Transcription activator-like effector (TALE).

MeSH terms

Cloning, Molecular
Gene Expression Regulation, Plant*
Gene Library
Gene Order
Gene Regulatory Networks*
Genetic Engineering
Genetic Vectors / genetics
Plants / genetics*
Plants / metabolism*
Promoter Regions, Genetic*
Transcription Activator-Like Effectors / metabolism*
Transcriptional Activation*

Substances

Transcription Activator-Like Effectors