GSTP1 and XRCC1 polymorphisms and DNA damage in agricultural workers exposed to pesticides

Mutat Res Genet Toxicol Environ Mutagen. 2017 Jul:819:20-25. doi: 10.1016/j.mrgentox.2017.05.005. Epub 2017 May 8.

Abstract

Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. These risks may be affected by polymorphisms of genes for glutathione transferase-dependent metabolism of pesticides and for DNA repair. We studied the prevalence of GSTP1 and XRCC1 polymorphisms and their possible correlation with DNA damage following prolonged pesticide exposure. DNA damage was estimated by the comet assay in peripheral blood samples from 51 pesticide-exposed workers and 50 controls. GSTP1 (105) and XRCC1 (399 and 194) genotypes were identified by restriction fragment length analysis. Individuals carrying theGSTP1 Ile-Ile or XRCC1399 Arg-Arg genotypes showed greater DNA damage than observed for other alleles.

Keywords: Comet assay; GSTP1; Pesticide exposure; Polymorphisms; XRCC1.

MeSH terms

  • Amino Acid Substitution / genetics
  • Comet Assay
  • DNA Damage* / genetics
  • DNA-Binding Proteins / genetics*
  • Farmers*
  • Genotype
  • Glutathione S-Transferase pi / genetics*
  • Humans
  • Male
  • Middle Aged
  • Occupational Exposure / adverse effects*
  • Pesticides / toxicity*
  • Polymorphism, Restriction Fragment Length*
  • X-ray Repair Cross Complementing Protein 1

Substances

  • DNA-Binding Proteins
  • Pesticides
  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human
  • GSTP1 protein, human
  • Glutathione S-Transferase pi