Visualizing adenosine-to-inosine RNA editing in single mammalian cells

Nat Methods. 2017 Aug;14(8):801-804. doi: 10.1038/nmeth.4332. Epub 2017 Jun 12.

Abstract

Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing levels vary.

MeSH terms

  • Adenosine / chemistry
  • Adenosine / genetics*
  • Cell Line
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Inosine / chemistry
  • Inosine / genetics*
  • Molecular Imaging / methods*
  • Neurons / metabolism*
  • Optical Imaging / methods
  • RNA Editing / genetics*

Substances

  • Inosine
  • Adenosine