Brachybacterium sp. CH-KOV3 isolated from an oil-polluted environment-a new producer of levan

Aleksandra Djurić; Gordana Gojgić-Cvijović; Dragica Jakovljević; Branka Kekez; Jovana Stefanović Kojić; Maija-Liisa Mattinen; Inka Elina Harju; Miroslav M Vrvić; Vladimir P Beškoski

doi:10.1016/j.ijbiomac.2017.06.034

Brachybacterium sp. CH-KOV3 isolated from an oil-polluted environment-a new producer of levan

Int J Biol Macromol. 2017 Nov;104(Pt A):311-321. doi: 10.1016/j.ijbiomac.2017.06.034. Epub 2017 Jun 8.

Authors

Aleksandra Djurić¹, Gordana Gojgić-Cvijović², Dragica Jakovljević², Branka Kekez³, Jovana Stefanović Kojić², Maija-Liisa Mattinen⁴, Inka Elina Harju⁵, Miroslav M Vrvić⁶, Vladimir P Beškoski³

Affiliations

¹ University of Belgrade, Faculty of Chemistry, Studentski trg 12-16 P.O. Box 158, 11001, Belgrade, Serbia; University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, 11001 Belgrade, Njegoševa 12, Serbia. Electronic address: adjuric@chem.bg.ac.rs.
² University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, 11001 Belgrade, Njegoševa 12, Serbia.
³ University of Belgrade, Faculty of Chemistry, Studentski trg 12-16 P.O. Box 158, 11001, Belgrade, Serbia.
⁴ Aalto University, School of Chemical Technology, Department of Forest Products Technology Bioproduct Chemistry, PO Box 16300, FI-00076, Aalto, Finland.
⁵ Clinical Microbiology Laboratory, Turku University Hospital, Finland.
⁶ University of Belgrade, Faculty of Chemistry, Studentski trg 12-16 P.O. Box 158, 11001, Belgrade, Serbia; University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, 11001 Belgrade, Njegoševa 12, Serbia.

PMID: 28602989
DOI: 10.1016/j.ijbiomac.2017.06.034

Abstract

Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500g/L of sucrose. The optimal temperature and pH were 28°C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC-MS analysis confirmed that the residues were d-fructofuranosyl residues with β-configuration, where fructose units are linked by β-2,6-glycosidic bonds, with β-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.

Keywords: Brachybacterium paraconglomeratum; Exopolysaccharides; Polluted environments.

MeSH terms

Actinobacteria / isolation & purification*
Actinobacteria / metabolism*
Environmental Pollution*
Fructans / biosynthesis*
Hydrogen-Ion Concentration
Petroleum / microbiology*
Temperature

Substances

Fructans
Petroleum
levan