Objectives: To build the viral integration sites map and verify the integration sites at or near the fragile site.
Methods: PCR method were applied to screen single HPV16 infected cervical carcinoma specimen and the viral integration sites in the specimens of cervical carcinomas and the cell line cells were detected by detection of integrated papillomavirus seguences by ligation-mediated PCR (DIPS-PCR).
Results: The integration site in cell line Siha was 13q22. The integration sites in the specimens of the cervical cancinomas were randomly located in chromosome 1, 3, 5, 6, 8, 10, 11, 13, 14, 15, 17, 19 as well as in X, but the sites were most likely at or near the chromosome fragile site. The location of the integration sites were at 1p35.1, 5p15.3, 10q24, 13q21-q22 and Xp11.4. By using the softwares CBS prediction servers and BDGP Neural Network Promoter prediction we found that the integration sites were located at the promoter like sequences.
Conclusions: DIPS-PCR can detect the viral integration sites, and the sites are at or near the chromosome fragile sites, and the integration sites may including the promoter to amplify the virus oncogene.
Keywords: Cervical carcinoma; DIPS-PCR; Fragile sites; High-risk human papillomavirus; Integration; Promoters.