Chaperone-Polymer-Assisted, Photodriven DNA Strand Displacement

Chembiochem. 2017 Aug 17;18(16):1568-1572. doi: 10.1002/cbic.201700202. Epub 2017 Jul 14.

Abstract

Photodriven DNA strand displacement by using a 2',6'-dimethylazobenzene-tethered strand and poly(l-lysine)-graft-dextran (PLL-g-Dex) as a chaperone is reported. Rapid strand displacement was reversibly induced by UV and visible-light irradiation without any toehold portion. To further improve the method, the concentration of PLL-g-Dex and the number of equivalents of the photoresponsive strand were optimised. Optimally, 64 % strand displacement was reversibly induced by alternating UV and visible-light irradiation.

Keywords: DNA; nanotechnology; photochemistry; polymers; substituent effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry
  • Azo Compounds / radiation effects*
  • DNA / chemistry
  • DNA / radiation effects*
  • Dextrans / chemistry*
  • Fluoresceins / chemistry
  • Fluorescent Dyes / chemistry
  • Isomerism
  • Nanotechnology
  • Nucleic Acid Hybridization
  • Polylysine / analogs & derivatives*
  • Polylysine / chemistry
  • Transition Temperature
  • Ultraviolet Rays
  • p-Dimethylaminoazobenzene / analogs & derivatives
  • p-Dimethylaminoazobenzene / chemistry

Substances

  • Azo Compounds
  • Dextrans
  • Fluoresceins
  • Fluorescent Dyes
  • poly(lysine)-graft-dextran
  • Polylysine
  • 4-(4-dimethylaminophenylazo)benzoic acid
  • 4-carboxyfluorescein
  • DNA
  • p-Dimethylaminoazobenzene