Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe3O4@CS/NHCS-Lip as a novel and promising nanobiocatalyst

Ali Baghban; Mohammad Heidarizadeh; Esmail Doustkhah; Sadegh Rostamnia; Parisa Fathi Rezaei

doi:10.1016/j.ijbiomac.2017.05.159

Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe₃O₄@CS/NHCS-Lip as a novel and promising nanobiocatalyst

Int J Biol Macromol. 2017 Oct:103:1194-1200. doi: 10.1016/j.ijbiomac.2017.05.159. Epub 2017 Jun 1.

Authors

Ali Baghban¹, Mohammad Heidarizadeh², Esmail Doustkhah³, Sadegh Rostamnia⁴, Parisa Fathi Rezaei⁵

Affiliations

¹ Department of Chemistry, Faculty of Science, Payame Noor University, PO Box 19395-3697, Tehran, Iran. Electronic address: Alibaghban@gmail.com.
² Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran; Department of Microbiology, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.
³ Young Researchers and Elite Club, Maragheh Branch, Islamic Azad University, Maragheh, Iran.
⁴ Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran. Electronic address: rostamnia@maragheh.ac.ir.
⁵ Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.

PMID: 28579461
DOI: 10.1016/j.ijbiomac.2017.05.159

Abstract

In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable Fe₃O₄@CS/NHCS₂H via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient.

Keywords: Covalently enzyme immobilization; Nanobiocatalyst; Pancreatic lipase.

MeSH terms

Animals
Biocatalysis*
Chitosan / chemistry*
Enzyme Stability
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism
Lipase / chemistry*
Lipase / metabolism*
Magnetite Nanoparticles / chemistry*
Models, Molecular
Pancreas / enzymology*
Swine
Thiocarbamates / chemistry*

Substances

Enzymes, Immobilized
Magnetite Nanoparticles
Thiocarbamates
Chitosan
Lipase