Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess

Roland Thele; Heidi Gumpert; Louise B Christensen; Peder Worning; Kristian Schønning; Henrik Westh; Thomas A Hansen

doi:10.1016/j.jgar.2017.05.007

Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess

J Glob Antimicrob Resist. 2017 Sep:10:1-2. doi: 10.1016/j.jgar.2017.05.007. Epub 2017 May 30.

Authors

Roland Thele¹, Heidi Gumpert¹, Louise B Christensen¹, Peder Worning¹, Kristian Schønning², Henrik Westh³, Thomas A Hansen⁴

Affiliations

¹ Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen Hvidovre, Denmark.
² Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen Hvidovre, Denmark; Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
³ Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen Hvidovre, Denmark; Faculty of Health Sciences, Institute of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.
⁴ Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen Hvidovre, Denmark. Electronic address: Thomas.arn.hansen@regionh.dk.

PMID: 28576740
DOI: 10.1016/j.jgar.2017.05.007

Abstract

The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sensitive to Danish-registered antibiotics although it had a fosA-like gene in the genome. There were nine contigs that aligned to a plasmid, and these contigs contained several heavy metal resistance gene homologues. Furthermore, a prophage was discovered in the genome. WGS represents an efficient tool for monitoring Kluyvera spp. and its role as a reservoir of multidrug resistance. Therefore, this susceptible K. intermedia genome has many characteristics that allow comparison of resistant K. intermedia that might be discovered in the future.

Keywords: Abscess; Antibiotics; Cadmium; Kluyvera intermedia; Resistance; WGS.

MeSH terms

Abscess / microbiology*
Anti-Bacterial Agents
Bacterial Proteins / genetics
DNA, Bacterial
Drug Resistance, Multiple, Bacterial / genetics
Enterobacteriaceae Infections / microbiology*
Humans
Kluyvera / drug effects
Kluyvera / genetics*
Kluyvera / isolation & purification*
Kluyvera / pathogenicity*
Microbial Sensitivity Tests
Plasmids
Whole Genome Sequencing
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA, Bacterial
beta-Lactamases