Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe

Xilei Xie; Fuyan Tang; Xiaoyan Shangguan; Shiyi Che; Jinye Niu; Yongsheng Xiao; Xu Wang; Bo Tang

doi:10.1039/c7cc03050a

Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe

Chem Commun (Camb). 2017 Jun 13;53(48):6520-6523. doi: 10.1039/c7cc03050a.

Authors

Xilei Xie¹, Fuyan Tang, Xiaoyan Shangguan, Shiyi Che, Jinye Niu, Yongsheng Xiao, Xu Wang, Bo Tang

Affiliation

¹ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China. tangb@sdnu.edu.cn wangxu@sdnu.edu.cn.

PMID: 28573306
DOI: 10.1039/c7cc03050a

Abstract

Lyso-TPFP presents lysosomal targetability and an acidic pH-activatable response toward formaldehyde. Thus, it exclusively visualizes lysosomal formaldehyde and is immune against it in neutral cytosol and other organelles. In addition, two-photon fluorescence imaging endows Lyso-TPFP with the capability of in situ tracking formaldehyde in live cells and animals.

MeSH terms

Animals
Cell Survival
Fluorescence
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Fluorescent Dyes / pharmacokinetics*
Formaldehyde / analysis*
HeLa Cells
Hep G2 Cells
Humans
Hydrogen-Ion Concentration
Lysosomes / metabolism*
Mice
Optical Imaging*
Photons*

Substances

Fluorescent Dyes
Formaldehyde