Identification of male-specific AFLP and SCAR markers in the dioecious plant Humulus scandens

Shu-Fen Li; Lian-Jun Wang; Chuan-Liang Deng; Wu-Jun Gao

doi:10.1016/j.mcp.2017.05.007

Identification of male-specific AFLP and SCAR markers in the dioecious plant Humulus scandens

Mol Cell Probes. 2017 Aug:34:68-70. doi: 10.1016/j.mcp.2017.05.007. Epub 2017 May 25.

Authors

Shu-Fen Li¹, Lian-Jun Wang², Chuan-Liang Deng¹, Wu-Jun Gao³

Affiliations

¹ College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, PR China.
² Institute of Food Corps, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, PR China.
³ College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, PR China. Electronic address: gaowujun1@163.com.

PMID: 28552829
DOI: 10.1016/j.mcp.2017.05.007

Abstract

In this study, 17 male-specific amplified fragment length polymorphism (AFLP) markers were identified between male and female Humulus scandens plants. BLAST analysis revealed that 7 of the 17 sex-linked sequences were highly similar to retrotransposons. Two stable male-specific sequence-characterized amplified regions (SCAR) markers were developed. These AFLP and SCAR markers are novel molecular probes that can be used efficiently to identify the genetic gender of H. scandens and may provide a basis for further investigations on the evolution of sex chromosomes.

Keywords: AFLP; Humulus scandens; Retrotransposable elements; SCAR; Sex-linked marker.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amplified Fragment Length Polymorphism Analysis / methods
Genes, Plant / genetics*
Genetic Markers / genetics*
Humulus / genetics*

Substances

Genetic Markers