Both non-covalent and covalent interactions were involved in the mechanism of detoxifying effects of persimmon tannin on Chinese cobra PLA2

Ying Zhang; Wei Zhu; Xiang-Yi Deng; Jin-Ming Peng; Chun-Mei Li

doi:10.1016/j.fitote.2017.05.009

Both non-covalent and covalent interactions were involved in the mechanism of detoxifying effects of persimmon tannin on Chinese cobra PLA₂

Fitoterapia. 2017 Jul:120:41-51. doi: 10.1016/j.fitote.2017.05.009. Epub 2017 May 26.

Authors

Ying Zhang¹, Wei Zhu¹, Xiang-Yi Deng¹, Jin-Ming Peng¹, Chun-Mei Li²

Affiliations

¹ College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
² College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China,; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China. Electronic address: lichmyl@mail.hzau.edu.cn.

PMID: 28552595
DOI: 10.1016/j.fitote.2017.05.009

Abstract

Persimmon tannin (PT) has been shown to inhibit snake venom activities and toxicities both in vitro and in vivo. To clarify the detoxifying mechanism of PT on snake venom, the interaction of characteristic structural elements of PT (EGCG, ECG, EGCG dimer and ECG dimer) and Chinese cobra phospholipase A₂ (PLA₂) was studied. The results revealed that except non-covalent bonds like hydrogen bonds, hydrophobic bonds and iron bonds were formed between PT and PLA₂, covalent interaction was also occurred. PT could bind with the key active residues of PLA₂, such as lysine, histidine, tryptophan and tyrosine, restraining their activity and disturbing the structure of PLA₂, thus showing detoxifying effects on snake venom.

Keywords: Covalent binding; Detoxifying mechanism; Persimmon tannin; Phospholipase A(2).

MeSH terms

Animals
Diospyros / chemistry*
Elapidae
Fruit / chemistry*
Molecular Structure
Phospholipases A2 / chemistry*
Snake Venoms / chemistry*

Substances

Snake Venoms
Phospholipases A2