EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

Marie Vandermies; Olivia Denies; Jean-Marc Nicaud; Patrick Fickers

doi:10.1016/j.mimet.2017.05.012

EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

J Microbiol Methods. 2017 Aug:139:161-164. doi: 10.1016/j.mimet.2017.05.012. Epub 2017 May 26.

Authors

Marie Vandermies¹, Olivia Denies¹, Jean-Marc Nicaud², Patrick Fickers³

Affiliations

¹ Microbial Processes and Interactions, TERRA Teaching and Research Center, University of Liège - Gembloux AgroBio Tech, Gembloux, Belgium.
² Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en Josas, France.
³ Microbial Processes and Interactions, TERRA Teaching and Research Center, University of Liège - Gembloux AgroBio Tech, Gembloux, Belgium. Electronic address: pfickers@ulg.ac.be.

PMID: 28552427
DOI: 10.1016/j.mimet.2017.05.012

Abstract

We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.

Keywords: Catabolic selectable marker; EYK1; Genome editing; Yarrowia lipolytica.

MeSH terms

Cloning, Molecular
DNA, Fungal / genetics
Gene Editing*
Gene Expression Regulation, Fungal
Genetic Markers
Phosphotransferases / genetics*
Phosphotransferases / metabolism
Saccharomyces cerevisiae / genetics
Tetroses / metabolism*
Transformation, Genetic
Yarrowia / enzymology*
Yarrowia / genetics*
Yarrowia / growth & development

Substances

DNA, Fungal
Genetic Markers
Tetroses
erythrulose
Phosphotransferases