RhoA and RhoC are involved in stromal cell-derived factor-1-induced cell migration by regulating F-actin redistribution and assembly

Mol Cell Biochem. 2017 Dec;436(1-2):13-21. doi: 10.1007/s11010-017-3072-3. Epub 2017 May 23.

Abstract

Stromal cell-derived factor-1 (SDF-1) signaling is important to the maintenance and progression of T-cell acute lymphoblastic leukemia by inducing chemotaxis migration. To identify the mechanism of SDF-1 signaling in the migration of T-ALL, Jurkat acute lymphoblastic leukemia cells were used. Results showed that SDF-1 induces Jurkat cell migration by F-actin redistribution and assembly, which is dependent on Rho activity. SDF-1 induced RhoA and RhoC activation, as well as reactive oxygen species (ROS) production, which was inhibited by Rho inhibitor. The Rho-dependent ROS production led to subsequent cytoskeleton redistribution and assembly in the process of migration. Additionally, RhoA and RhoC were involved in SDF-1-induced Jurkat cell migration. Taken together, we found a SDF-1/CXCR4-RhoA and RhoC-ROS-cytoskeleton pathway that regulates Jurkat cell migration in response to SDF-1. This work will contribute to a clearer insight into the migration mechanism of acute lymphoblastic leukemia.

Keywords: Cytoskeleton; Migration; ROS; Rho; SDF-1.

MeSH terms

  • Actins / metabolism*
  • Cell Movement*
  • Chemokine CXCL12 / metabolism*
  • Cytoskeleton / metabolism
  • Enzyme Activation
  • Humans
  • Jurkat Cells
  • Reactive Oxygen Species / metabolism
  • Signal Transduction*
  • rhoA GTP-Binding Protein / metabolism*
  • rhoC GTP-Binding Protein / metabolism*

Substances

  • Actins
  • CXCL12 protein, human
  • Chemokine CXCL12
  • Reactive Oxygen Species
  • RHOA protein, human
  • RHOC protein, human
  • rhoA GTP-Binding Protein
  • rhoC GTP-Binding Protein