Electrospun polymer scaffolds modified with drugs for tissue engineering

Abstract

The purpose of this paper was to fabricate nanofibrous scaffolds containing ossein-hydroxyapatite complex (osteogenon) to mimic the native bone extracellular matrix. Polylactide (PLDL) and polycaprolactone (PCL) were used to prepare scaffolds using electrospinning. Unfortunately, both of these biodegradable polymers have poor cell recognition sites leading to poor cell affinity and adhesion, therefore, based on our previous experience, osteogenon-drug was used at the stage of fibers forming by electrospinning. We have compare the physicochemical parameters and mechanical properties of PLDL/osteo and PCL/osteo scaffolds as well as an osteogenon-drug influence on the microstructure of electrospun materials produced for potential application in bone tissue engineering. We have investigated the effect of the microstructure and the chemical composition of electrospun materials on adhesion, proliferation and morphology as well as on the process of differentiation of bone cells. The use of osteogenon improved mineralization, cell adhesion and the rate of cell differentiation.

Keywords: Drug; Electrospinning; PCL; PLDL; Scaffolds; Tissue engineering.