Does arbuscular mycorrhizal fungus affect cadmium uptake and chemical forms in rice at different growth stages?

Na Luo; Xing Li; Ao Yu Chen; Li Jun Zhang; Hai Ming Zhao; Lei Xiang; Quan Ying Cai; Ce Hui Mo; Ming Hung Wong; Hui Li

doi:10.1016/j.scitotenv.2017.05.047

Does arbuscular mycorrhizal fungus affect cadmium uptake and chemical forms in rice at different growth stages?

Sci Total Environ. 2017 Dec 1:599-600:1564-1572. doi: 10.1016/j.scitotenv.2017.05.047. Epub 2017 May 18.

Authors

Na Luo¹, Xing Li¹, Ao Yu Chen¹, Li Jun Zhang¹, Hai Ming Zhao¹, Lei Xiang¹, Quan Ying Cai¹, Ce Hui Mo², Ming Hung Wong³, Hui Li⁴

Affiliations

¹ Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, PR China.
² Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, PR China. Electronic address: tchmo@jnu.edu.cn.
³ Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, PR China; Consortium on Environment, Health, Education and Research (CHEER), Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, Hong Kong, SAR, PR China.
⁴ Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, PR China. Electronic address: lihuizsu@126.com.

PMID: 28531965
DOI: 10.1016/j.scitotenv.2017.05.047

Abstract

Effects of the arbuscular mycorrhizal fungus (AMF) - Rhizophagus intraradices (a mix of root pieces, mycelium pieces and spores) on the temporal variation of Cd uptake and chemical forms in rice at four growth periods (tillering, jointing, flowering, and ripening stages) were investigated in soil added with 0, 2 and 10mgCdkg^-1. Results showed that the interactions amongst rice growth stages, soil Cd concentrations and mycorrhizal inoculation had significant effects (P˂0.001) on root biomass, straw and root Cd concentrations, and straw Cd chemical forms in rice. Root colonization rates fluctuated with growth stages, reaching its peak at jointing stage and then decreasing at flowering and ripening stages. AMF increased the grain yield in rice plant grown in soil added with 10mgCdkg^-1, whereas no effect was found in soil added with 2mgCdkg^-1. In soil added with 2mgCdkg^-1, the concentrations of ethanol and d-H₂O extractable Cd at flowering stage was significantly reduced in mycorrhizal treatments, which subsequently induce less Cd accumulation in grains due to the positive correlations between ethanol or d-H₂O extractable Cd and grain Cd concentrations at flowering stage. In soil added with 10mgCdkg^-1, AMF significantly elevated the proportions of NaCl extractable Cd at ripening stage which also lead to the reduced grain Cd concentrations, since there was a negative correlation between the percentage of NaCl extractable Cd and grain Cd concentration at this stage. Our study indicated that flowering and ripening stages were important periods for AMF to limit the grain Cd concentrations in rice, when grown in Cd-contaminated soil.

Keywords: Growth period; Heavy metal; Oryza sativa L; Rhizophagus intraradices.

MeSH terms

Cadmium / metabolism*
Glomeromycota
Mycorrhizae*
Oryza / metabolism*
Oryza / microbiology*
Plant Roots / microbiology
Soil
Soil Pollutants / metabolism*

Substances

Soil
Soil Pollutants
Cadmium