Development of genipin-crosslinked fucoidan/chitosan-N-arginine nanogels for preventing Helicobacter infection

Yu-Hsin Lin; Kun-Ying Lu; Ching-Li Tseng; Jui-Yu Wu; Chien-Ho Chen; Fwu-Long Mi

doi:10.2217/nnm-2017-0055

Development of genipin-crosslinked fucoidan/chitosan-N-arginine nanogels for preventing Helicobacter infection

Nanomedicine (Lond). 2017 Jun;12(12):1491-1510. doi: 10.2217/nnm-2017-0055. Epub 2017 May 19.

Authors

Yu-Hsin Lin^{1

2}, Kun-Ying Lu³, Ching-Li Tseng⁴, Jui-Yu Wu^{3

5}, Chien-Ho Chen⁶, Fwu-Long Mi^{3

5

7}

Affiliations

¹ Department of Biological Science & Technology, China Medical University, Taichung 40402, Taiwan, ROC.
² Department of Bioinformatics & Medical Engineering, Asia University, Taichung 41354, Taiwan.
³ Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
⁴ Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 11031, Taiwan, ROC.
⁵ Department of Biochemistry & Molecular Cell Biology, School of medicine, College of medicine, Taipei Medical University, Taipei City 11031, Taiwan.
⁶ School of Medical Laboratory Science & Biotechnology, College of Medical Science & Technology, Taipei Medical University, Taipei, Taiwan.
⁷ Graduate Institute of Nanomedicine & Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.

PMID: 28524785
DOI: 10.2217/nnm-2017-0055

Abstract

Aim: This study aims to validate the anti-Helicobacter pylori efficacy of amoxicillin-loaded nanoparticles and nanogels with pH-responsive and site-specific drug release properties against H. pylori infection.

Materials & methods: Genipin-crosslinked low molecular weight fucoidan/chitosan-N-arginine nanogels (FCSA) were prepared for targeted delivery of amoxicillin to the site of H. pylori infected AGS gastric epithelial cells.

Results: The negatively charged nanogels (n-FCSA) adhered to H. pylori and exhibited pH-responsive drug release property to reduce cytotoxic effects in H. pylori infected AGS cells.

Conclusion: These in vitro findings suggest that n-FCSA nanogels are potential carriers for H. pylori specific delivery of antibacterial agents, and provide the basis for further studies on the clinical use of the nanogels.

Keywords: antibacterial; chitosan; drug delivery; fucoidan; nanogels; nanoparticles.