Biphasic Supramolecular Self-Assembly of Ferric Ions and Tannic Acid across Interfaces for Nanofilm Formation

Abstract

Cell nanoencapsulation provides a chemical tool for the isolation and protection of living cells from harmful, and often lethal, external environments. Although several strategies are available to form nanometric films, most methods heavily rely on time-consuming multistep processes and are not biocompatible. Here, the interfacial supramolecular self-assembly and film formation of ferric ions (Fe^III ) and tannic acid (TA) in biphasic systems is reported, where Fe^III and TA come into contact each other and self-assemble across the interface of two immiscible phases. The interfacial nanofilm formation developed is simple, fast, and cytocompatible. Its versatility is demonstrated with various biphasic systems: hollow microcapsules, encasing microbial or mammalian cells, that are generated at the water-oil interface in a microfluidic device; a cytoprotective Fe^III -TA shell that forms on the surface of the alginate microbead, which then entraps probiotic Lactobacillus acidophilus; and a pericellular Fe^III -TA shell that forms on individual Saccharomyces cerevisiae. This biphasic interfacial reaction system provides a simple but versatile structural motif in materials science, as well as advancing chemical manipulability of living cells.

Keywords: biphasic systems; cell nanoencapsulation; metal-organic complexes; nanofilms; supramolecular self-assembly.