CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus

Xiaoyun Dong; Yingli Jin; Di Ming; Bangbang Li; Haisi Dong; Lin Wang; Tiedong Wang; Dacheng Wang

doi:10.1016/j.mimet.2017.05.008

CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus

J Microbiol Methods. 2017 Aug:139:79-86. doi: 10.1016/j.mimet.2017.05.008. Epub 2017 May 15.

Authors

Xiaoyun Dong¹, Yingli Jin², Di Ming³, Bangbang Li¹, Haisi Dong³, Lin Wang⁴, Tiedong Wang⁵, Dacheng Wang⁶

Affiliations

¹ Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, China.
² Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, China. Electronic address: jinyingli@jlu.edu.cn.
³ Department of Biochemistry and Molecular Biology, College of Animal Science, Jilin University, Changchun, China.
⁴ Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address: wanglin1020@jlu.edu.cn.
⁵ Department of Biochemistry and Molecular Biology, College of Animal Science, Jilin University, Changchun, China. Electronic address: wangtd@jlu.edu.cn.
⁶ Department of Biochemistry and Molecular Biology, College of Animal Science, Jilin University, Changchun, China. Electronic address: wangdc@jlu.edu.cn.

PMID: 28522389
DOI: 10.1016/j.mimet.2017.05.008

Abstract

The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.

Keywords: CRISPR interference; Gene knockdown; Staphylococcus aureus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats
Gene Expression*
Gene Knockdown Techniques / methods*
Staphylococcus aureus / genetics*