Objective: To establish the method of colloidal gold immunochrom atographic assay for detecting cadmium ions rapidly. Methods: The anti-cadmium ion monoclonal antibody-gold conjugate was labeled on the binding pad, cadmium ion hapten and goat anti-mouse IgG were coated on nitrocellulose membrane as the detection line (T line) and quality control line (C line) respectively. The sample pad, colloidal gold bonding pad, nitrocellulose membrane and absorption pad were orderly assembled on the PVC board to cut into a test paper strip. The qualitative results of the assay were visualized in color. Results: When detecting the human urine cadmium ions, the results were tested qualitativly within 15 minutes. The detection limit was 30 μg/L. No cross-reactivity with other heavy metal ions. The test paper strip could be stored at 4 ℃ for 3 months. Conclusion: The method has the advantages of low cost, strong specificity, good stability and reliable results, and is suitable for rapid screening of cadmium poisoning of enterprise and occupational health.
目的： 建立人体尿中镉离子的胶体金免疫层析快速检测方法。 方法： 将抗镉离子单克隆抗体-金标复合物标记在结合点垫上，镉离子半抗原和羊抗鼠IgG分别包被于硝酸纤维素膜上作为检测线（T线）和质控线（C线），将样品垫、胶体金结合垫、硝酸纤维素膜和吸收垫在聚氯乙烯（PVC）板上依次组装好切割成试纸条，样品经过层析，以T线颜色深浅直观显示检测的定性结果。 结果： 该方法镉离子检出限为30 μg/L，15 min内可定性检测结果，与其他重金属离子无交叉反应，试纸条4 ℃可保存3个月。 结论： 该方法成本低、特异性强、稳定性好，结果可靠，适用于企业及职业卫生重金属镉中毒的快速筛查。.
Keywords: Cadmium; Gold colloid; Immunochromatography; Test paper strip.