Single particle labeling of RNA virus in live cells

Xiaohui Liu; Ting Ouyang; Hongsheng Ouyang; Linzhu Ren

doi:10.1016/j.virusres.2017.05.007

Single particle labeling of RNA virus in live cells

Virus Res. 2017 Jun 2:237:14-21. doi: 10.1016/j.virusres.2017.05.007. Epub 2017 May 12.

Authors

Xiaohui Liu¹, Ting Ouyang¹, Hongsheng Ouyang¹, Linzhu Ren²

Affiliations

¹ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, China.
² Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, China. Electronic address: renlz@jlu.edu.cn.

PMID: 28506790
DOI: 10.1016/j.virusres.2017.05.007

Abstract

Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the current knowledge of interactions between RNA viruses (especially influenza viruses, immunodeficiency viruses, and Flaviviruses) and host cellular structures, obtained using single particle labeling techniques combined with real-time fluorescence microscopy. Single particle labeling provides an easy system for understanding the RNA virus life cycle.

Keywords: Live cell; RNA virus; Single particle labeling.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Host-Pathogen Interactions*
Microscopy, Fluorescence / methods*
RNA Viruses / physiology*
Staining and Labeling / methods*
Virology / methods*
Virus Replication*