Sublocalization of Cytochrome b6f Complexes in Photosynthetic Membranes

Abstract

It is well established that the majority of energy-converting photosynthetic protein complexes in plant thylakoid membrane are nonhomogenously distributed between stacked and unstacked membrane regions. Yet, the sublocalization of the central cytochrome b₆f complex remains controversial. We present a structural model that explains the variation in cytochrome b₆f sublocalization data. Small changes in the distance between adjacent membranes in stacked grana regions either allow or restrict access of cytochrome b₆f complexes to grana. If the width of the gap falls below a certain threshold, then the steric hindrance prevents cytochrome b₆f access to grana. Evidence is presented that the width of stromal gap is variable, demonstrating that the postulated mechanism can regulate the lateral distribution of the cytochrome b₆f complexes.

Keywords: cytochrome b(6)f complex; grana; photosynthesis; thylakoid membrane.