Abstract
We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 104UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.
Keywords:
Brazil; Carbapenemases; Enterobacteriaceae; OXA-48; OXA-48 K-SeT.
Copyright © 2017 Elsevier B.V. All rights reserved.
MeSH terms
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Bacteriological Techniques
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Blood / microbiology*
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Blood Culture*
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Chromatography, Affinity / methods*
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / growth & development
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Enterobacteriaceae / isolation & purification
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Enterobacteriaceae Infections / microbiology
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Escherichia coli Proteins / isolation & purification
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Humans
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Rectum / microbiology*
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beta-Lactamases / analysis*
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beta-Lactamases / isolation & purification
Substances
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Escherichia coli Proteins
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beta-Lactamases
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beta-lactamase OXA-48, E coli
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oxacillinase