Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay

J Microbiol Methods. 2017 Aug:139:92-94. doi: 10.1016/j.mimet.2017.05.003. Epub 2017 May 5.

Abstract

We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 104UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.

Keywords: Brazil; Carbapenemases; Enterobacteriaceae; OXA-48; OXA-48 K-SeT.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques
  • Blood / microbiology*
  • Blood Culture*
  • Chromatography, Affinity / methods*
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / growth & development
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae Infections / microbiology
  • Escherichia coli Proteins / isolation & purification
  • Humans
  • Rectum / microbiology*
  • beta-Lactamases / analysis*
  • beta-Lactamases / isolation & purification

Substances

  • Escherichia coli Proteins
  • beta-Lactamases
  • beta-lactamase OXA-48, E coli
  • oxacillinase