Abstract
New fluorogenic sensors with suitable kinetic parameters and sensitivity have been developed for the determination of sphingosine-1-phosphate lyase activity in cell lysates. The probe RBM148 can be efficiently loaded into cationic liposomes and used to determine S1PL activity in intact cells.
MeSH terms
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Aldehyde-Lyases / chemistry
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Aldehyde-Lyases / metabolism*
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Animals
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Fluorescent Dyes / chemistry*
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Gene Deletion
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Gene Expression Regulation, Enzymologic
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HEK293 Cells
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Humans
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Mice
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Molecular Structure
Substances
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Fluorescent Dyes
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Aldehyde-Lyases
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sphingosine 1-phosphate lyase (aldolase)