The first fluorogenic sensor for sphingosine-1-phosphate lyase activity in intact cells

Chem Commun (Camb). 2017 May 11;53(39):5441-5444. doi: 10.1039/c7cc01659j.

Abstract

New fluorogenic sensors with suitable kinetic parameters and sensitivity have been developed for the determination of sphingosine-1-phosphate lyase activity in cell lysates. The probe RBM148 can be efficiently loaded into cationic liposomes and used to determine S1PL activity in intact cells.

MeSH terms

  • Aldehyde-Lyases / chemistry
  • Aldehyde-Lyases / metabolism*
  • Animals
  • Fluorescent Dyes / chemistry*
  • Gene Deletion
  • Gene Expression Regulation, Enzymologic
  • HEK293 Cells
  • Humans
  • Mice
  • Molecular Structure

Substances

  • Fluorescent Dyes
  • Aldehyde-Lyases
  • sphingosine 1-phosphate lyase (aldolase)